Abstract: Diarrhea is the major health problem in under five children. Worldwide, about 1.5 million children die of diarrhea, while 38% deaths occur in Asia. About 2 million episodes of diarrhea occur each year in India. 6.6 million deaths among children aged 28 days to 5 years: The present descriptive study aimed to assess knowledge regarding diarrhea among the mothers of under five children in selected area of Aurangabad city. 30 mothers of under-five children were included by nonprobability purposive sampling technique. Results of the study indicate that, the majority 23(76.66%) of the mothers of under five children had moderately adequate knowledge on diarrhea, followed by 4(13.33%) mothers had adequate knowledge and 3(10%) had inadequate knowledge regarding diarrhea.